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亚硒酸钠对帕金森病大鼠的黑质形态学与纹状体多巴胺含量及血液水平的影响
曾朝蓉,岳冬,孙伟,张昕,张铭,姚晓梅,熊义英,胡延春,陈正礼
0
(成都中医药大学附属四川省八一康复中心;动物疫病与人类健康四川省重点实验室?四川农业大学动物医学院)
摘要:
目的 探讨亚硒酸钠对帕金森病大鼠的黑质形态学、纹状体多巴胺含量及血液水平的影响。方法 选取 8只大鼠作为对照组(I组),其余40只连续7 d腹腔注射MPTP建立PD模型。选建模成功的24只PD大鼠,随机分 为MPTP组(II组)、MPTP十0.05 mg/kg Se组(皿组)、MPTP+0.1 mg/kg Se组(W组),每组8只。Ⅲ组与IV组用不同 剂量亚硒酸钠灌胃30 d。用Nissl染色法sTUNEL法观察各组黑质DA能神经元形态学变化并用ELISA法检测纹状 体多巴胺含量,用血常规、血生化检测各组血液指标变化。目的 探讨亚硒酸钠对帕金森病大鼠的黑质形态学、纹状体多巴胺含量及血液水平的影响。方法 选取 8只大鼠作为对照组(I组),其余40只连续7 d腹腔注射MPTP建立PD模型。选建模成功的24只PD大鼠,随机分 为MPTP组(II组)、MPTP十0.05 mg/kg Se组(Ⅲ组)、MPTP+0.1 mg/kg Se组(W组),每组8只。皿组与IV组用不同 剂量亚硒酸钠灌胃30 d。用Nissl染色法sTUNEL法观察各组黑质DA能神经元形态学变化并用ELISA法检测纹状 体多巴胺含量,用血常规、血生化检测各组血液指标变化。结果 与I组相比,口组的尼氏小体数量显著减少、黑质细 胞凋亡数量显著增加、纹状体内DA含量显著降低(P<0. 05);DBIL含量显著降低,WBC、Neu、Lym、Mon、UA均显著 增高(PV0.05);与I[组相比,[D[组尼氏小体的数量显著增加、细胞凋亡数显著减少、DA含量显著升高(P<0. 05);TP. ALB. AST.CHOL.Urea.UA含量显著降低(P<0. 05), HGB、ALP、GLU含量显著升高(P<0. 05) ; IV组尼氏小体数量 有所恢复、细胞凋亡数有所减少、DA含量所有增加但差异均不显著;ALB\Urea含量显著降低頒(P<0.05)。结论剂量 为0. 05 mg/kg亚硒酸钠可以减轻PD大鼠神经元变性,抑制DA含量的降低,对PD大鼠的肝功、肾功、.血糖等指标有显 著性影响,而血常规指标除HGB外无显著性变化。 与I组相比,口组的尼氏小体数量显著减少、黑质细 胞凋亡数量显著增加、纹状体内DA含量显著降低(P<0. 05);DBIL含量显著降低,WBC、Neu、Lym、Mon、UA均显著 增高(P<0.05);与II组相比,Ⅲ组尼氏小体的数量显著增加、细胞凋亡数显著减少、DA含量显著升高(P<0. 05);TP. ALB. AST.CHOL.Urea.UA含量显著降低(P<0. 05), HGB、ALP、GLU含量显著升高(P<0. 05) ; IV组尼氏小体数量 有所恢复、细胞凋亡数有所减少、DA含量所有增加但差异均不显著;ALB\Urea含量显著降低頒<0.05)。结论 剂量为0. 05 mg/kg亚硒酸钠可以减轻PD大鼠神经元变性,抑制DA含量的降低,对PD大鼠的肝功、肾功、.血糖等指标有显 著性影响,而血常规指标除HGB外无显著性变化。
关键词:  帕金森病;大鼠模型;亚硒酸钠  黑质神经形态;多巴胺含量;血液指标
DOI:
基金项目:四川省科技计划项目(2017JY0314)
Effects of sodium selenite on morphology of substantia nigra, dopamine content in striatum and blood level in rats with Parkinsons disease
ZENG Chaorong,YUE Dong,SUN Wei,Zhang Xin,Zhang Ming,YAO Xiaomei,XIONG Yiying,HU Yanchun,CHEN Zhengli
(The Affiliated Sichuan Ba-Yi Rehabilitation Center of Chengdu University of TCM;Key Laboratory of Animal Disease and Human Health in Sichuan Province,Veterinary Medicine College of Sichuan Agricultural University)
Abstract:
Objective To investigate the effects of sodium selenite on morphology of substantia nigra % dopamine content in striatum and blood level of Parkinsons Disease (PD) model rats. Methods 8 rats were selected as the control group (group I). The remaining 40 rats were injected with MPTP for 7 days to establish PD model, 24 PD rats were randomly divided into MPTP group ( R group) , MPTP+ 0. 05 mg/kg Se group (皿 group) , MPTP+ 0. 1 mg/kg Se group (IV group) , 8 rats in each group. Group III and group IV were given different doses of sodium selenite for 30 days. Morphological changes of DA neurons in substantia nigra were observed by Nissl staining and TUNEL, and dopa mine content in striatum was detected by ELISA. The changes of blood indexes in each group were detected by blood routine and blood biochemistry. Results Compared with group I, the number of Nissl bodies in group II decreased signif icantly, the number of apoptotic cells in substantia nigra increased significantly (P<0. 05). The content of DA in stria turn decreased significantly (P<0. 05), DBIL was significantly decreased? WBC? Neu? Lym, Mon and UA were signifi cantly increase (P<0. 05). Compared with group II? the number of Nissl bodies ? the number of apoptotic cells and the content of DA in group III increased significantly (P<0. 05), The contents of TP, ALB, AST? CHOL, Urea and UA decreased significantly (P<0. 05) , while the contents of HGB, ALP and GLU increased significantly (P<0. 05). In group IV, the number of Nissl bodies recovered, the number of apoptotic cells decreased and the content of DA increased, but the difference was not significant. The contents of ALB and Urea in group IV decreased significantly (P< 0. 05), Conclusion Sodium selenite at the dose of 0. 05mg/kg can alleviate neuron degeneration in PD rats, inhibit the reduction of DA content, and have a significant impact on liver function, kidney function? blood glucose and other indica tors in PD rats.
Key words:  Parkinson's disease  Rat model  Sodium selenite  Neu romo r p ho logy of the substantia nigra  Dopa mine content  Blood parameters

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