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芫根提取液抗疲劳作用的实验研究
骆芷寒,彭博,王禹蒙,代泽咏,江舟,成姝婷,肖静,王正荣
0
(四川大学华西基础医学与法医学院,国家卫生健康委员会时间生物学重点实验室)
摘要:
【摘要】 目的 探讨高原作物芫根提取液的抗疲劳作用。方法 选用雄性ICR小鼠作为实验动物,采用随机分组的方法将小鼠分为芫根组、红景天组和空白对照组3组,每组各24只。芫根组小鼠通过灌胃法使其连续摄入芫根提取液,红景天组小鼠灌胃等量的红景天口服液,空白对照组小鼠以同样的方法灌胃灭菌去离子水,3组均持续灌胃14天,每天1次。分别在灌胃第7天和第14天测定小鼠的力竭游泳时间、全血血糖水平、血清乳酸水平和血清乳酸脱氢酶活性。取小鼠小肠组织提取mRNA并测序,测序结果进行差异表达分析,同时通过相关分析预测软件预测对与乳酸脱氢酶(LDH)的主要亚基LDHA相互作用的基因。 结果 芫根组和红景天组小鼠的血糖水平在灌胃第7天和第14天均无明显变化(P>0.05)。与空白对照组相比,芫根组和红景天组小鼠的力竭游泳时间在灌胃第7天无明显差异(P>0.05),在灌胃第14天均显著延长(P<0.01),但芫根组与红景天组小鼠之间的力竭游泳时间比较差异无统计学意义(P>0.05)。与空白对照组比较,芫根组小鼠的血清乳酸水平在灌胃第7天和第14天均显著降低(P<0.01);而红景天组小鼠的血清乳酸水平仅在灌胃第14天才出现显著降低(P<0.01);芫根组小鼠的血清乳酸水平在灌胃第7天明显低于红景天组(P<0.05),但在灌胃第14天两组比较无明显差异(P>0.05)。与空白对照组相比,芫根组和红景天组小鼠的血清乳酸脱氢酶活性在灌胃第7天升高(P<0.05),在灌胃第14天显著升高(P<0.01)。芫根组与空白对照组相比,差异表达基因中HIF1A 和ABCC9的表达水平呈显著上调(P<0.05),而编码LDH关键亚基LDHA的基因在芫根组中表达下调,但差异无统计学意义(P>0.05)。分析转录组测序结果后,预测了五个与乳酸脱氢酶A(LDHA)相互作用的基因。结论 芫根提取液具有提高血清乳酸脱氢酶活性、降低小鼠血清乳酸含量的作用,但对小鼠血糖水平无影响;芫根对乳酸脱氢酶的表达量和活性的影响可能是分别通过HIF1A和ABCC9实现的;长期服用芫根提取液可通过提高血清乳酸脱氢酶活性而加快血清乳酸的清除,从而达到与红景天相似的抗疲劳效果。
关键词:  芫根提取液  抗疲劳  乳酸  乳酸脱氢酶  力竭游泳时间
DOI:
基金项目:国家自然科学基金;四川省科技厅重点项目
Study on the anti fatigue effect of Brassica rapa L extract
LUO Zhihan,PENG Bo,WANG Yumeng,DAI Zeyong,JIANG Zhou,CHENG Shuting,XIAO Jing,WANG Zhengrong
(NHC Key Laboratory of Chronobiology, West China School of Basic Medical Sciences & Forensic Medicine, Sichuan University)
Abstract:
【Abstract】 Objective To study the antifatigue effect and mechanism of Brassica rapa L extract of highaltitude crops. Methods Male ICR mice were used as experimental animals, and the mice were randomly divided into three groups. Brassica rapa L extract was given to the mice by continuous gavage for 14 days, rhodiola group mice and the control group mice were intragastrically administrated by rhodiola oral liquid andsterilized deionized water in the same manner. The fatigue swimming time of the mice was respectively measured on the 7th and 14th day of the gavage, then testing the blood glucose levels, serum lactate levels and serum lactate dehydrogenase levels. Extracting the mice small intestine tissue,and sent to bio company for sequencing. The sequencing results were analyzed for differential expression. At the same time, lactate dehydrogenase A, the major subunit of lactate dehydrogenase (LDH), were predicted by correlation analysis prediction software. Results In the entire experiment, the blood glucose level of the Brassica rapa L group and rhodiola group did not change significantly (P>0.05). Compared with the control group, the antifatigue swimming time of the mice in the Brassica rapa L group was prolonged with the prolongation of time, and it significantly prolonged at 14th days (P<0.01). The serum lactic acid content significantly decreased on the 7th day (P<0.01), in the meantime, serum lactate dehydrogenase activity also increased significantly on the 7th day (P<0.01); However, compared with the Rhodiola group mice, there was no significant difference. The Rhodiola group mice also showed a significant increase in antifaigue swimming time (P<0.01) and a significant decrease in serum lactic acid content at 14th days(P<0.01), and significantly increased serum lactate dehydrogenase activity (P<0.05). After analyzing transcriptome sequencing results, predicted five interacting genes of lactate dehydrogenase A(LDHA). It is estimated that the Brassica rapa L extract may be regulated by HIF1A and ABCC9 for the activity of lactic acid dehydrogenase by consulting the literature analysis. Conclusion Brassica rapa L extract has the effect of increasing serum lactate dehydrogenase activity and reducing serum lactic acid content of mice, but has no effect on blood glucose levels in mice. The effect of Brassica rapa L on the expression and activity of lactate dehydrogenase may be achieved by HIFA and ABCC9 separately; Longterm use of Brassica rapa L extract can accelerate clearance of serum lactic acid by increasing serum lactate dehydrogenase activity, consequently achieving similar antifatigue effects to Rhodiola.
Key words:  Brassica rapa L  Anti fatigue  Lactic acid  Lactate dehydrogenase  Exhausted swimming time

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