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长链非编码RNA MIR29B1在系统性红斑狼疮中的表达及意义
郭敏,苏江,刘毅
0
(四川大学华西医院风湿免疫科;成都市第七人民医院风湿免疫科;四川省医学科学院·四川省人民医院·电子科技大学医学院风湿免疫科)
摘要:
【摘要】 目的 探讨长链非编码RNA(lncRNA)MIR29B1在系统性红斑狼疮(SLE)中的表达及意义。方法 收集2016年~2018年12月于四川大学华西医院就诊的85例SLE患者作为SLE组,另选取同时期77例健康体检者为对照组。qRT-PCR检测两组血清中MIR29B1的表达水平。使用小干扰RNA(siRNA)敲除MIR29B1并转染至THP-细胞(siMIR29B1组),另转染siRNA作为siCtrol组作为siCtrol组。Rt-PCR和蛋白质印迹法分别检测MIR29B1和IL-10的水平。结果 SLE患者MIR29B1表达异常增加;沉默MIR29B1显著降低了SLE患者中IL-10的表达。结论 MIR29B1是SLE发病机制中的关键调节因子,这为治疗SLE提供了潜在的新靶点。
关键词:  系统性红斑狼疮  长链非编码RNA  MIR29B1  自身免疫性疾病  发病机制
DOI:
基金项目:国家重点研发计划(2016YFC0906201);四川大学华西医院学科卓越发展1·3·5工程项目(ZYGD18015)
Expression and clinical significance of serum LncRNA MIR29B1 in patients with systemic lupus erythematosus
GUO Min,SU Jiang,LIU Yi
(Department of Rheumatology and Immunology, West China Hospital, Sichuan University;Department of Rheumatology and Immunology, Chengdu Seventh People′s Hospital;Department of Rheumatology and Immunology, Sichuan Academy of Medical Sciences & Sichuan Provincial People′s Hospital, The Affiliated Hospital of the University of Electronic Science and Technology)
Abstract:
【Abstract】 Objective n this study, we aimed to explore the contribution of the lncRNA MIR29B1 to the pathogenesis of SLE. Methods The expression levels of MIR29B1 were measured by quantitative PCR. Small interfering RNA (siRNA) was then used to knock down the expression of MIR29B1 in order to determine the role of MIR29B1 in the expression levels of IL10 in SLE patients. Results We found MIR29B1 expression was abnormally increased in SLE patients. Silencing MIR29B1 significantly reduced the expression of IL10 in SLE patients.Conclusion Our results demonstrate that MIR29B1 is the key regulatory factor in the pathogenesis of SLE and provides potentially novel target for therapeutic intervention.
Key words:  Systemic lupus erythematosus  LncRNA MIR29B1  MIR29B1  Autoimmune disease  Pathogenesis

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