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复方当归注射液对肌成纤维细胞bcl2和bax的表达影响
王达,闵倩,章乐怡
0
(长沙市第一医院口腔科;长沙市口腔医院)
摘要:
【摘要】目的 探讨复方当归注射液对肌成纤维细胞bcl2和bax表达影响及治疗口腔黏膜下纤维性变的可能性。方法 体外培养人口腔黏膜成纤维细胞,并使其转分化肌成纤维细胞(MFB),分为对照组和5、10、20、40 mg/mL组5组。采用western blot法检测不同时间5组bcl2,bax蛋白水平。结果 5~40 mg/mL的复方当归注射液作用于肌成纤维细胞24、48、72 h后bax表达随时间逐渐增加,bcl2表达则逐渐减少(P<0.05);各浓度组与对照组相比及各浓度组bcl2和bax相比差异均具有统计学意义(P<0.01)。结论 复方当归注射液可能通过影响bcl2和bax的表达来促进MFB的凋亡,从而具有治疗口腔黏膜下纤维性变的可能性。
关键词:  复方当归注射液  肌成纤维细胞  bcl 2  bax
DOI:
基金项目:
The effect of compound angelica injection on expression of bcl2 and bax of myofibroblast
WANG Da,MIN Qian,ZHANG Leyi
(The First Hospital of Changsha;Changsha Stomatology Hospital)
Abstract:
【Abstract】Objective To investigate the effect of compound angelica injection on expression of Bcl2 and Bax of myofibroblast and provide the theoretical basis for the treatment of oral submucous fibrosis.Methods Human oral mucosa fibroblasts were cultured in vitro and transformed into myofibroblasts (MFB), which were divided into control group and 5, 10, 20, 40 mg / ml group. Western blot was used to detect the levels of bcl 2 and Bax protein in five groups at different times. Results After 5-40 mg/mL compound angelica injection on myofibroblasts for 24, 48 and 72 hours, the expression of Bax increased gradually with time, while the expression of Bcl2 decreased gradually (P<005). The difference of bcl 2 and Bax in each concentration group was statistically significant (P<001).Conclusion Compound angelica injection may promote the apoptosis of MFB by affecting the expression of bcl 2 and Bax, so it has the possibility of treating oral submucous fibrosis.
Key words:  Compound Angelica Injection  Myofibroblast  Bcl 2 protein  Bax protein

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