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花姜酮对Hela细胞株细胞生物学特性的影响
吴玲姣,邓晓杨,徐蕾,邓洁,朱晓莺,吴海燕
0
(成都医学院第一附属医院妇科)
摘要:
【摘要】目的 探究花姜酮对Hela细胞株增殖、迁移、侵袭及凋亡的影响。方法 购买人宫颈癌Hela细胞株,进行培养并分为空白组、花姜酮5 μmol/L组、花姜酮10 μmol/L组、花姜酮20 μmol/L组4组。空白组使用常规细胞培养液培养,其他三组分别使用相对应浓度的花姜酮进行培养。观察4组细胞的增殖、迁移、侵袭、凋亡情况及上皮细胞间充质(EMT)相关蛋白相对表达量。结果 24、48、72 h花姜酮20 μmol/L组细胞增殖率低于花姜酮5 μmol/L组、花姜酮10 μmol/L组及空白组(P<005);24、48、72 h花姜酮20 μmol/L组细胞凋亡率高于花姜酮5 μmol/L组、花姜酮10 μmol/L组及空白组(P<005);花姜酮20 μmol/L组细胞迁移、侵袭数均低于花姜酮5 μmol/L组、花姜酮10 μmol/L组及空白组(P<005);花姜酮20 μmol/L组细胞波形蛋白(Vimentin)、组蛋白甲基转移酶同源序列2增强子(EZH2)相对表达量均低于花姜酮5 μmol/L组、花姜酮10 μmol/L组空白组;花姜酮20 μmol/L组细胞α连环蛋白(αcat)、上皮性钙粘蛋白(Ecadherin)相对表达量均高于花姜酮5 μmol/L组、花姜酮10 μmol/L组及空白组(P<005)。结论 花姜酮能够促进HeLa细胞株的凋亡,抑制Hela细胞株的增殖、迁移及侵袭能力,其能力呈现浓度依赖。
关键词:  花姜酮  Hela细胞  增殖  凋亡  迁移
DOI:
基金项目:四川省教育厅资助课题重点项目(17ZA0128)
Effects of zingiberenone on proliferation, migration, invasion and apoptosis of HeLa cell line
WU Lingjiao,DENG Xiaoyang,XU Lei,DENG Jie,ZHU Xiaoying,WU Haiyan
(Department of Gynaecology, The First Affiliated Hospital of Chengdu Medical College)
Abstract:
【Abstract】Objective To investigate the effects of zingiberenone on proliferation, migration, invasion and apoptosis of HeLa cell line. Methods HeLa cell line of human cervical cancer was cultured and divided into blank group, 5μmol/L group, 10μmol/L group and 20μmol/L group. Cell proliferation, migration, invasion, apoptosis and relative expression of EMTrelated proteins were observed. Results At 24h, 48h and 72h, the cell proliferation rate in the 20 μmol/L group was lower than that in the 5 μmol/L group, the 10 μmol/L group and the blank group (P<005). At 24h, 48h and 72h, the apoptosis rate of the 20 μmol/L group was higher than that of the 5 μmol/L group, the 10 μmol/L group and the blank group (P<005). The cell migration and invasiveness of 20 μmol/L group were all lower than those of 5 μmol/L group, 10 μmol/L group and blank group (P<005). The relative expression levels of Vimentin and Enhancer of zeste homolog 2 (EZH2) in human cells in the 20 μmol/L group were all lower than those in the 5 μmol/L group, the 10 μmol/L group, and the blank group. The relative expression levels of αcat and ecadherin were all higher than those of 5 μmol/L group, 10 μmol/L group and blank group (P<005). Conclusion Zingiberenone can promote the apoptosis of HeLa cells and inhibit the proliferation and migration of HeLa cells. Invasiveness and capacity were concentration dependent.
Key words:  Zingiberenone  Hela cell  Cell proliferation  Apoptosis  Migration

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