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斑蝥素酸镁对人星形胶质瘤细胞U-251MG生长、凋亡和侵袭的影响
李从进,刘锋,王路,郑涛
0
(安康市中心医院神经外科;西安国际医学中心)
摘要:
目的 探讨斑蝥素酸镁对人星形胶质瘤细胞U251MG生长、凋亡和侵袭的影响及机制。方法 体外培养人星形胶质瘤细胞U251MG,分为空白组(Control组)、低剂量斑蝥素酸镁组(Canthardin 5μM组)、中剂量斑蝥素酸镁组(Canthardin 10μM组)和高剂量斑蝥素酸镁组(Canthardin 20μM组)。采用克隆形成实验检测细胞增殖情况;Hocest染色检测细胞凋亡情况;Transwell法检测细胞侵袭情况;Western blot 检测相关蛋白表达情况。结果 平板克隆实验结果显示,斑蝥素酸镁可以明显抑制人星形胶质瘤细胞U251MG的增殖,且呈剂量依赖性(P<0.05);Hocest染色结果显示,斑蝥素酸镁可以明显促进人星形胶质瘤细胞的凋亡,且呈剂量依赖性(P<0.05);Transwell小室实验显示,斑蝥素酸镁可以显著抑制细胞的侵袭,且呈剂量依赖性(P<0.05);Western blot 检测结果显示,斑蝥素酸镁处理后,人星形胶质瘤细胞中PCNA蛋白、VEGF蛋白、Bax蛋白及p-AKT蛋白表达均显著降低(均P<0.05),Caspase3蛋白、Bcl2蛋白表达显著升高(P<0.05),AKT总蛋白表达无显著差异(P>0.05)。 结论 斑蝥素酸镁可以通过调控Akt信号通路抑制人星形胶质瘤细胞U-251MG的增殖和侵袭,并促进其凋亡,且在一定浓度范围内呈剂量依赖性。
关键词:  人星形胶质瘤细胞  斑蝥素酸镁  细胞凋亡  细胞侵袭
DOI:
基金项目:陕西省自然科学基础研究计划项目(2017JQ5034)
Effects and mechanism of magnesium canthardin on growth, apoptosis and invasion of human astroglioma cell U-251MG
LI Congjin,LIU Feng,WANG Lu,ZHENG Tao
(Department of Neurosurgery, Ankang Central Hospital;Xi′an International Medical Center)
Abstract:
Objective To investigate the effects and mechanisms of magnesium canthardin on growth, apoptosis and invasion of human astroglioma cell U251MG. Methods Human astroglioma cells U251MG were cultured in vitro. They were divided into blank group (Control), lowdose magnesium cantharidin group (Canthardin 5 μM), mediumdose magnesium cantharidin group (Canthardin 10 μM) and highdose magnesium cantharidin group (Canthardin 10μM). Cell proliferation was detected by colony formation assay. Apoptosis was detected by Hocest staining. Cell invasion was detected by Transwell method. The expression of related proteins were detected by Western blot. Results The results of plate cloning experiments showed that magnesium cantharidin could significantly inhibit proliferation of human astroglioma cell U251MG, showing dosedependence (P<0.05). Hocest staining results showed magnesium cantharidin could significantly promote apoptosis of human astroglioma cells, showing dosedependence (P<0.05). Transwell chamber experiments showed that magnesium cantharidin could significantly inhibit cell invasion, showing dosedependence (P<0.05). The test results of Western blot showed expression of PCNA protein, VEGF protein, Bax protein and pAKT protein in human astroglioma cells were significantly decreased after processed by magnesium cantharidin (P<0.05), while expression of Caspase3 protein and Bcl2 protein were significantly increased (P<0.05). There was no significant difference in expression of total AKT protein (P>0.05).〖WTHZ〗Conclusion Magnesium cantharidin can inhibit proliferation and invasion of human astroglioma cell U-251MG and promote its apoptosis by regulating Akt signaling pathway, showing dosedependence in certain concentration range.
Key words:  Human astroglioma  Magnesium cantharidin  Apoptosis  Cell invasion

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