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三七皂苷R1对创伤性休克大鼠心脏功能、肺组织损伤及免疫失衡的实验研究
王琦,李海荣,李佩阳,校建波,王伯良
0
(空军军医大学第二附属医院急诊科)
摘要:
目的 探讨三七皂苷R1对创伤性休克大鼠心脏功能、肺组织损伤及免疫失衡的影响。 方法 将45只清洁级SD大鼠随机分为对照组、模型组和三七皂苷R1高、中、低剂量治疗组,共5组,每组9只。模型组和高、中、低剂量治疗组采用股骨中上段闭合性骨折及股动脉放血构建创伤性休克大鼠模型,对照组只做创伤不做休克;各组均进行生理盐水复苏,高、中、低剂量治疗组大鼠分别接受 50、100、200 mg/kg的三七皂苷R1灌胃,1次/d,连续灌胃7d;对照组和模型组灌胃等体积剂量的生理盐水。比较各组大鼠的心脏功能;采用ELISA试剂盒法分析各组大鼠血清中肌酸激酶(CK)、肌酸激酶同工酶(CK-MB)、Mb(肌红蛋白)及炎症因子的表达水平;通过HE染色和肺组织湿/干比分析各组大鼠肺组织病理损伤;westernblot分析肺组织中ERK1/2、pERK1/2、p65及p-p65水平。〖HTH〗结果 高、中、低剂量治疗组大鼠的MAP、HR、LVSP指数及血清中IL-10水平明显升高,血清中Mb、cTc I、CK-MB、TNF、IL-1β、iNOS水平及肺组织的湿/干比值显著降低(P<0.05);病理学结果显示,高、中、低剂量治疗组大鼠肺组织损伤显著改善;Westernblot结果显示,高、中、低剂量治疗组大鼠肺组织中ERK1/2/pERK1/2表达比及pp65的表达水平显著降低,且均呈现出明显的剂量依耐性降低(P<0.05) 结论 高剂量的三七皂苷R1能够通过阻断ERK1/2及NF-kB信号通路调节炎症因子的表达水平,改善因创伤性休克引起的大鼠心脏功能和肺功能损伤。
关键词:  三七皂苷R1  创伤性休克  心脏功能  肺组织损伤
DOI:
基金项目:陕西省自然科学基金(2017Jc2-16)
Experimental study on effects of notoginsenoside R1 on cardiac function, lung tissue injury and immune imbalance in rats with traumatic shock
WANG Qi,LI Hairong,LI Peiyang,XIAO Jianbo,WANG Boliang
(Department of Emergency, The Second Affiliated Hospital of Air Force Military Medical University)
Abstract:
Objective To explore effects of notoginsenoside R1 on cardiac function, lung tissue injury and immune imbalance in rats with traumatic shock. Methods 45 SD rats were randomly divided into control group, model group, high, medium and lowdose notoginsenoside R1 treatment group. Model group and treatment groups were given closed fracture of upper middle femur and femoral artery bloodletting to establish rat models of traumatic shock, while control group was only treated with trauma and no shock. The rats in each group were resuscitated with normal saline. The treatment groups were treated with 50, 100 and 200 mg/kg notoginsenoside R1 for intragastric administration (once/d, continuous intragastric administration for 7d). The control group and model group were intragastrically administered with an equal volume of normal saline. The cardiac function was compared among all groups. The expression levels of serum creatine kinase (CK), creatine kinase isoenzyme (CK-MB), Mb (myoglobin) and inflammatory factors in each group were analyzed by ELISA kit. The pathological injury of lung tissues was analyzed by HE staining and lung tissue wet/dry ratio. The levels of ERK1/2, pERK1/2, p65 and pp65 in lung tissues were analyzed by western blot.Results MAP, HR and LVSP, and level of serum IL-10 in notoginsenoside R1 treatment group were significantly increased, while levels of serum Mb, cTcI, CK-MB, TNF, IL-1β and iNOS, and lung tissue wet/dry ratio were significantly decreased (P<0.05). The pathological results showed that lung tissue injury was significantly improved. Westernblot results showed that expression ratio of ERK1/2/pERK1/2 and expression level of pp65 in rat lung tissues were significantly decreased (P<0.05), and they showed significant decreases in dosedependence (P<0.05). Conclusion Highdose notoginsenoside R1 can improve cardiac function and lung function injury in rats caused by traumatic shock by blocking ERK1/2 and NF-kB signaling pathways, and regulate expression levels of inflammatory factors.
Key words:  Notoginsenoside R1  Traumatic shock  Cardiac function  Lung tissue injury

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