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脓毒症心肌损害新生大鼠模型的建立
易玲,李雅斐,刘忠强,卿璐,乔莉娜,母得志
0
(四川大学华西第二医院儿科·出生缺陷与相关妇儿疾病教育部重点实验室)
摘要:
【摘要】 目的 建立一种具有良好稳定性及重复性的能模拟新生儿脓毒症(NS)心肌损害的新生大鼠动物模型,为研究NS致病机制及防治提供模型动物。方法 选取出生10天的健康雄性SD新生大鼠54只,随机分为正常对照组、假手术组(Sham组)、盲肠结扎穿孔术组(CLP组),每组各18只。通过观察血培养菌落生长,采用酶联免疫吸附法(ELISA)对血清肌钙蛋白I(cTNI)浓度、心肌组织肿瘤坏死因子α(TNFα)和白细胞介素1β(IL1β)含量进行检测,光镜下观察HE染色后心肌组织的病理变化。结果 正常对照组和Sham组新生大鼠的血培养结果均为阴性,CLP组新生大鼠的血培养中检出以大肠杆菌为主的菌落。正常对照组新生大鼠的血清cTNI浓度、心肌组织TNFα和IL1β含量和Sham组比较差异无统计学意义(P>0.05);CLP组新生大鼠的血清cTNI浓度、心肌组织TNFα和IL1β含量较Sham组明显增加(P<0.05)。正常对照组和Sham组的心肌组织HE染色示心肌细胞均未出现炎性改变,但CLP组心肌细胞周围出现了炎性细胞浸润等病理改变。结论 通过盲肠结扎穿孔可建立较为理想的脓毒症心肌损害新生大鼠模型。
关键词:  脓毒症  心肌损害  新生大鼠  模型  盲肠结扎穿孔术
DOI:
基金项目:国家自然科学基金;四川省科技计划项目;四川省卫生厅科研课题
Establish a neonatal rat model of sepsis induced myocardial damage by cecal ligation and puncture
YI Ling,LI Yafei,LIU Zhongqiang,QING Lu,QIAO Lina,MU Dezhi
(Department of Pediatrics, Key Laboratory of Obstetric & Gynecologic and Pediatric Diseases and Birth Defects of Ministry of Education, West China Second University Hospital)
Abstract:
【Abstract】 Objective To establish a stable and reproducible neonatal rat model of sepsisinduced myocardial damage and provide model animals for studying the pathogenesis and prevention of neonatal sepsis. Methods 54 10day age male SD neonatal rats were randomly divided into normal control group,sham group and cecal ligation and puncture (CLP) group. Colony growth was observed by blood culture. Serum cardiac troponin I (cTNI), antitumor necrosis factorα (TNFα) and interleukin1β (IL1β) concentration of myocardial tissue were detected by ELISA. Change of myocardial tissue was observed under light microscope after hematoxylin eosin staining. Results Negative blood culture results were found in the normal control group and sham group. A variety of pathogenic bacteria especially Escherichia coli were detected in CLP group. There was no significance between the concentration of cTNI, TNFα and IL1β in the normal control group and the sham group (P>0.05). The concentration of cTNI, TNFα and IL1β were significantly higher in the CLP group than in the sham group (P<0.05). According to results of hematoxylin eosin staining, there were no inflammatory changes in cardiac myocytes in normal control group and sham group, but inflammatory cell infiltration around cardiac myocytes was observed in CLP group. Conclusion For establishing the neonatal rat model of sepsisinduced myocardial damage, CLP is a preferable choice in our research.
Key words:  Sepsis  Myocardial damage  Neonatal rat  Model  Cecal ligation and puncture

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