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超声评价微创横向主动脉缩窄小鼠模型心脏重构变化
雷迁,魏新川,徐金东,柴云飞,曾思,徐广民,范丹
0
(四川省医学科学院·四川省人民医院·电子科技大学附属医院麻醉科;广东省人民医院·广东省医学科学院麻醉科)
摘要:
目的 采用超声评价微创横向主动脉缩窄(MITAC)小鼠模型的心脏结构和功能改变,探讨该模型模拟心力衰竭病理生理过程的稳定性和有效性。方法 以雄性C57BL/6小鼠为研究对象,随机分为假手术组和MITAC组,每组12只。采用改良的胸骨上入路技术暴露主动脉弓,假手术组不钳夹主动脉,MITAC组以钛夹缩窄主动脉。使用小动物超声系统检查,术后1周测量主动弓血流速度,1、4、8周测量左心室收缩末、舒张末内径和左心室后壁舒张末厚度,并计算左心室射血分数和缩短分数。对两组超声检查结果进行统计分析。结果 所有小鼠手术过程顺利,假手术组术后无死亡,MITAC组术后死亡1只(83%)。MITAC组与假手术组相比,术后1周主动弓血流速度明显加快(P<0.001),术后4周室壁厚度增加(P<0.01),术后8周左心室显著增大、收缩功能明显减低(P<0.001)。结论 超声作为一种无创检查手段,能动态评估以及证实MITAC技术能够稳定、有效地建立小鼠心力衰竭模型。
关键词:  横向主动脉缩窄  超声心动图  心肌肥厚  心力衰竭  动物模型
DOI:
基金项目:国家自然科学基金(81500281)
Echocardiography evaluation of heart remodelling in the mouse model of minimally invasive transverse aortic constriction
LEI Qian,WEI Xinchuan,XU Jindong,CHAI Yunfei,ZENG Si,XU Guangmin,FAN Dan
(Department of Anesthesiology, Sichuan Academy of Medical Sciences, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China;Department of Anesthesiology, Guangdong General Hospital, Guangdong Academy of Medical Sciences)
Abstract:
Objective To evaluate the construction and heart function changes in the mouse model of minimally invasive transverse aortic constriction (MITAC) with echocardiography and discuss the stability and effectiveness of this model for simulating the pathophysiology of heart failure. Methods The 8-10 weeks male C57BL/6 mice were randomly divided into Sham group and MITAC group. The aortic arch was isolated through modified suprasternal approach. The aortic arch was clamped with titanium clip in the MITAC group, but not in the Sham group. An echo system for small animals was used for measuring the blood flow velocity at aortic arch in the 1stweek after surgery, as well as left ventricular end-systolic internal dimension (LVIDs), left ventricular enddiastolic internal dimension (LVIDd) and left ventricular posterior wall enddiastolic thickness (LVPWd) in the 1st, 4thand 8thweek after surgery. Meanwhile, the ejection fraction and fractional shortening was calculated. Statistical analysis was performed for echo data in these two groups. Results Both groups enrolled 12 mice and the surgeries were successful for all the mice. Except for one mice (83%) in the MITAC group died after surgery, other mice were alive till the 8th week after surgery. Comparing MITAC group with Sham group, the blood flow velocity at aortic arch in the 1stweek after surgery increased significantly (P<0.001). The ventricular wall thickness increased obviously in the 4th week after surgery (P<0.01). The left ventricular internal dimensions were enlarged and systolic function decreased significantly in the 8thweek after surgery (P<0.001). Conclusion As a noninvasive examination technique, echocardiography played an important role in the dynamic evaluation for MITAC mouse model and identification for stable and effective establishment of heart failure model.
Key words:  Transverse aortic constriction  Echocardiography  Myocardial hypertrophy  Heart failure  Animal model

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