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丁香苷对H/R心肌细胞的保护作用及其机制研究
石美晶,师幸伟,石珍,夏慧兰,林立
0
(长江航运总医院;华中科技大学同济医学院附属同济医院)
摘要:
【摘要】 目的 探讨基质金属蛋白酶抑制剂(RECK)、基质金属蛋白酶9(MMP9)在三阴性乳腺癌(TNBC)组织、癌旁组织中的表达及与TNBC临床病理的关系。方法 选择绵阳市中心医院乳腺外科2011年5月~2013年7月经病理证实为TNBC的72例石蜡标本及同患者癌旁组织,利用免疫组化组织芯片技术检测其RECK、MMP9蛋白的表达。结果 RECK在TNBC组织及癌旁组织中阳性表达率分别为4306%、8056%(P=0000)。TNBC中RECK的表达与临床分期(P=0009)、组织学分级(P=0010)、腋窝淋巴结转移情况(P=0000)相关。MMP9在TNBC组织及癌旁组织中阳性表达率分别为625%、1528%(P=0000)。TNBC中MMP9的表达与肿瘤大小(P=0017)、临床分期(P=0001)、组织学分级(P=0001)、腋窝淋巴结转移状况(P=0001)、Ki67表达情况(P=0034)相关。TNBC中RECK与MMP9表达呈负相关(r=0195,P<005)。结论 RECK的表达缺失与MMP9过度表达与TNBC浸润、转移有关,有望成为TNBC的预后指标,并且有可能成为TNBC治疗的靶点。
关键词:  丁香苷  缺氧复氧  凋亡  氧化应激  炎症  AMPK
DOI:
基金项目:武汉市卫生计生委科研项目(WX15D30)
Expression of RECK and MMP 9 in tissue microarray of triple negative breast cancer and its clinical significance
SHI Meijing,SHI Xingwei,SHI Zhen,XIA Huilan,LIN LI
(General Hospital of Yangtze River Shipping;Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology)
Abstract:
【Abstract】 Objective To measure the roles of syringin (SYR) on hypoxia/reoxygenation (H/R)treated cardiomyocytes and possible mechanims. Methods Cultured cardiomyocytes were assigned into control group, H/R group and H/R+SYR group. Three hours of hypoxia and 3h of reoxygenation were performed to cause H/R injury. Cell viability, LDH release, apoptosis, ROS, inflammation and molecular alternations were systematically detected. Moreover, the specific AMPK inhibitor (Compound c) was added in mechanistic measurement. Results In comparison with the control group, H/R resulted in the reduced cell viability and increased LDH release, followed by the worsened ROS, apoptosis and inflammation. However, above parameters induced by H/R injury were significantly attenuated by SYR treatment. Mechanistic experiment showed SYR administration enhanced the level of pAMPK, and blocking AMPK with the specific inhibitor blunted H/Rrepressing effects of SYR. Conclusion SYR attenuates myocardial H/R injury in part via a AMPKdependent manner.
Key words:  Syringin  H/R  ROS  Apoptosis  Inflammation  AMPK

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