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应用iTRAQ技术对支原体肺炎患儿血清差异蛋白的定量分析
彭珊璐,李凤艳,李博,戴勇,王凯
0
(深圳市坪山区人民医院坪环社区康服务中心·深圳市坪山区人民医院中心实验室;深圳市人民医院儿科·深圳市人民医院临床医学研究中心)
摘要:
【摘要】 目的 应用同位素相对标记与绝对定量技术(iTRAQ)分析支原体肺炎患儿血清蛋白,筛选出差异蛋白并行其功能分析。方法 采用强阳离子交换色谱分析法对8种不同同位素标记的iTRAQ和液相色谱法以及串联质谱分析支原体肺炎组(MPP组)和健康对照组血清蛋白并通过Mascot软件分析和GO分析表达差异的蛋白。 结果 与对照组相比,MPP组中共鉴定出有定量信息的蛋白质66个,包括52个上调蛋白和14个下调蛋白。生物信息学分析显示差异表达的蛋白主要参与急性期反应、急性炎症反应、氧气输送、空气运输、过氧化氢分解过程、细胞氧化解毒、凝血、及细胞解毒等过程,这些表达差异的蛋白中选择6个显著表达差异作为候选蛋白,分别是上调蛋白SAA、CRP、HBB、APOM和下调蛋白APOC3、GPX3。 结论 本研究发现这6种蛋白在小儿支原体肺炎过程中发挥重要作用,可能作为该病潜在的临床筛查生物标志物。
关键词:  iTRAQ  支原体肺炎  差异蛋白  生物标志物
DOI:
基金项目:深圳市科技计划项目(JCYJ20150403093323880;JCYJ20150403101146298)
Analysis of differential serum proteomic in children with mycoplasma pneumonia using iTRAQ technology
PENG Shanlu,LI Fengyan,LI Bo,DAI Yong,WANG Kai
(Pinghuan Community Health Service Center and Central Lab, Shenzhen Pingshan People’s Hospital;Department of Pediatrics and Clinical medical Research Center, Shenzhen People’s Hospital)
Abstract:
【ABSTRACT】 Objective To analyze the proteins in serum of mycoplasma pneumonia and find the differentially expressed proteome with isobaric tags for relative and absolute Quantification (iTRAQ) technology. Methods The total proteins in serum samples from patients with mycoplasma pneumonia and samples of healthy controls were analyzed by eightplex iTRAQ added with strong cation exchange choematography and liquid chromatography coupled with tandem mass spectrometry. The differentially expressed proteins were identified by Mascot and GO. Results 66 differentially expressed proteins were identified. 52 proteins were upregulation and 14 proteins were downregulation. Bioinformatics analysis revealed that differential expression proteins were mainly related to the acutephase response, acute inflammatory response, oxygen transport, gas transport, hydrogen, peroxide catabolic process, cellular oxidant detoxification, blood coagulation, wound healing, coagulation, and cellular detoxification process. Among of them, there were 5 proteins significant differentially expressed, including upregulation expressed proteins SAA, CRP, HBB and APOM; downregulation expressed proteins APOC3 and GPX3. Conclusion This study finds that six kinds of protein in children with mycoplasma pneumonia play an important role in the process, promisingly as the disease clinical screening of potential biomarkers.
Key words:  iTRAQ  Mycoplasma pneumonia  Differentially expressed protein  Biomarker

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