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Toll样受体在介导危重型手足口病炎性反应中的作用
朱磊,方代华,齐共健,钮明杨,祁伯祥
0
(徐州市儿童医院)
摘要:
【摘要】 目的 研究Toll样受体在介导危重型手足口病(HFMD)炎性反应中的作用。方法 选择2016年07月~2017年03月收治的47例危重型手足口病患儿(危重型组)为研究对象,另选取同期感染科收治30例普通型HFMD作为疾病对照组,门诊健康体检30例儿童为健康对照组,采集外周静脉血,分离单个核细胞(PBMCs),测定TLR2、3、4、7、8、9水平及血清TNFα、IFNγ、IL6水平。结果 三组受试者PBMCs中TLR3、TLR4、TLR7和TLR8 mRNA表达水平差异有统计学意义(P<005),TLR2和TLR9 mRNA表达水平差异无统计学意义(P>005)。其中危重型手足口病组PBMCs中TLR3、TLR4、TLR7和TLR8 mRNA表达水平显著高于其他两组,健康对照组最低(P<005)。三组受试者血清中TNFα、IFNγ、IL6表达水平有显著性差异;危重型手足口病患儿血清中TNFα、IFNγ、IL6表达水平显著高于其他两组,健康对照组最低(P<005)。Pearson相关性分析显示,PBMCs中TLR3、TLR4、TLR7和TLR8 mRNA水平与血清中TNFα、IFNγ、IL6水平呈正相关(P<005)。结论 危重型手足口病TLR3、TLR4、TLR7和TLR8表达明显增高,进而激活相关信号通路,介导机体的炎性反应。
关键词:  危重型手足口病  Toll样受体  细胞因子
DOI:
基金项目:徐州市科技计划项目(KC16SL140);徐州市儿童医院计划项目(XEYKT1403)
The inflammatory response of Toll like receptor in mediating severe hand foot and mouth disease
ZHU Lei,FANg Daihua,QI Gongjian,NIU Mingyang,QI Boxiang
(Xuzhou Children's Hospital)
Abstract:
【Abstract】 Objective To investigate the inflammatory response of Tolllike receptor in mediating severe handfootand mouth disease (HFMD). Methods Twentyseven children with severe HFMD hospitalized in Intensive Care Unit of Xuzhou Children's Hospital were enrolled in the study from July 2016 to January 2017. Thirty children with common HFMD were selected as disease control, and thirty healthy children as healthy control. Peripheral vein blood samples were collected from each individual, and used to isolate peripheral blood mononuclear cells (PBMCs) for mRNA extraction and sera for measuring levels of tumor necrosis factor α, interferon γ, interleukin 6. Results The expression levels of TLR3,TLR4, TLR7 and TLR8 mRNA in PBMCs of children with severe HFMD were significantly higher than those in the other children. The lowest values were found in the healthy control group (P<005). The expression levels of TLR2 and TLR9 mRNA were not significantly different among the groups(P>005). Additionally, the expression levels of TNF α, IFN γ and IL 6 in the serum of the children in the severe group were significantly higher than those in the other two groups, and the lowest values were again found in the healthy control group (P<005). Pearson correlation analysis showed that the TLR3, TLR4, TLR7 and TLR8 mRNA levels in PBMCs were positively correlated with the TNF α, IFN γ, IL 6 levels in the serum (P<005). Conclusion The expression levels of TLR3, TLR4, TLR7 and TLR8 is higher, which then leads to the activation downstream signaling and affect the inflammatory response of the organism.
Key words:  Severe hand foot and mouth  Toll like receptor  Cytokines

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