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长链非编码RNA H19靶向Bcl2的表达调控宫颈癌细胞的侵袭转移行为
相海云,姚露露,张婕
0
(临沂市中心医院产科;中南大学湘雅附一院妇科)
摘要:
【摘要】 目的 探讨长链非编码RNAH19调控宫颈癌细胞的侵袭转移行为及其机制。方法 qPCR检测H19在宫颈癌组织和不同宫颈癌细胞株中的表达情况;分析H19和宫颈癌临床患者的临床病理学参数之间的关系;免疫共沉淀实验检测H19与Bcl2之间的相互作用;划痕愈合实验检测抑制H19后宫颈癌细胞的迁移能力的变化情况,Transwell侵袭实验检测抑制H19后宫颈癌细胞的侵袭行为的变化情况;Western blotting检测lncRNA H19对Ecadherin和Bcl2蛋白的调控情况。结果 与正常宫颈组织相比,宫颈癌组织中H19的表达水平相对上调,与其他宫颈癌细胞株相比,HeLa细胞中H19表达最高;免疫共沉淀实验证实H19能调控Bcl2的表达;抑制H19的表达后可以抑制宫颈癌细胞的增殖和侵袭能力;H19的表达与宫颈癌的病理分期相关以及淋巴结转移情况有关,随着分期越高,H19在宫颈癌组织中表达越高,淋巴结转移的患者中H19的表达也相对较高;抑制H19后宫颈癌细胞的迁移和侵袭受到相应的抑制,抑制H19后Bcl2和ECadherin蛋白的表达。结论 H19可以调控Bcl2的表达影响宫颈癌细胞的生物学行为。
关键词:  H19  宫颈癌  Bcl2表达调控  侵袭转移
DOI:
基金项目:山东省卫生厅基金(2014WS0290)
Long chain non coding RNA H19 targeting Bcl2 regulates the invasion and metastasis of cervical cancer cells
XIANG Haiyun,YAO Lulu,ZHANG Jie
(Department of Obstetrics, Linyi Central Hospital;Department of Gynaecology, The First Affiliated Hospital of Central South University Xiangya School of Hunan)
Abstract:
【Abstract】 Objective To investigate the effect of longchain noncoding RNAH19 on the invasion and metastasis of cervical cancer cells and its mechanism. Methods qPCR was used to detect the expression of H19 in cervical cancer tissues and different cervical cancer cell lines. The relationship between H19 and clinicopathological parameters in clinical patients with cervical cancer was analyzed. Immunoprecipitation assay was used to detect the interaction between H19 and Bcl2. Scrap healing test was used to detect the changes of migration ability of cervical cancer cells after H19, Transwell invasion assay was used to detect the changes of invasive behavior of cervical cancer cells after H19. Western blotting was used to detect the regulation of Ecadherin and Bcl2 protein by lncRNA H19. Results Compared with normal cervical tissues, the expression of H19 in cervical cancer tissues was higher than that in other cervical cancer cells. The expression of H19 was the highest in HeLa cells compared with other cervical cancer cell lines. Immunoprecipitation experiments confirmed that H19 could regulate the expression of Bcl2. Inhibition of H19 expression could inhibit the proliferation of cervical cancer cells and invasive ability. The expression of H19 was related to the pathological stage of cervical cancer and the lymph node metastasis.The higher the expression of H19, the higher the expression of H19 in cervical cancer tissues, and the higher expression of H19 in patients with lymph node metastasis.Conclusion H19 can regulate the expression of Bcl2 and affect the biological behavior of cervical cancer cells.
Key words:  H19  Cervical cancer  Bcl2  Transwell

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