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miR-363通过靶向HMGA2调控非小细胞肺癌的细胞生长与侵袭
梁忆华,钟海波,张敏
0
(暨南大学医学院附属广州红十字会医院呼吸内科)
摘要:
【摘要】 目的 探讨miR363通过靶向高迁移率蛋白A2(High mobility protein A1,HMGA2)调控非小细胞肺癌的细胞生长与侵袭行为及其作用机制。方法 qPCR检测非小细胞肺癌和癌旁正常组织、不同细胞株中miR363的表达;分析miR363的表达与非小细胞肺癌临床病理学参数之间的关系; CCK8细胞增殖实验检测miR363对非小细胞肺癌细胞增殖能力的影响;流式细胞术检测miR363对非小细胞肺癌细胞周期的影响;划痕愈合实验和Transwell侵袭实验检测抑制miR363后非小细胞肺癌细胞迁移和侵袭能力的变化;双荧光素酶报告基因检测miR363与HMGA2的相互作用。结果 与癌旁正常组织比较,非小细胞肺癌组织中miR363表达明显增高;非小细胞肺癌细胞A549中miR363的表达水平最高;抑制miR363的表达后非小细胞肺癌细胞增殖能力减弱,促进其凋亡行为;抑制miR363的表达后,A549细胞的迁移和侵袭能力受到相应的抑制;miR363能与HMGA2的3’ UTR特异性结合,调控HMGA2的表达活性。结论 miR363在非小细胞肺癌的发生发展过程中起着重要作用,可通过靶向调节HMGA2的表达影响非小细胞肺癌细胞的生长和侵袭。
关键词:  非小细胞肺癌  miR-363  HMGA2  侵袭行为
DOI:
基金项目:国家自然科学基金(81100042)
miR-363 regulates the growth and invasion of non small cell lung cancer cells by targeting HMGA2
LIANG Yihua,ZHONG Haibo,ZHANG Min
(Department of Respiratory Medicine, Guangzhou Red Cross Society Hospital Affiliated to Jinan University Medical School)
Abstract:
【Abstract】 Objective To investigate the effect of miR363 on the growth and invasion of nonsmall cell lung cancer cells by targeting HMGA2 and its mechanism. Methods qPCR was used to detect the expression of miR363 in nonsmall cell lung cancer and adjacent normal tissues. The relationship between the expression of miR363 and clinicopathological parameters of nonsmall cell lung cancer was analyzed. The proliferation of nonsmall cell lung cancer cells was detected by CCK8 cell proliferation assay. The effect of miR363 on cell cycle of nonsmall cell lung cancer was detected by flow cytometry. Scratch healing assay and Transwell invasion assay were used to detect the migration and invasion ability of miR363 cells after nonsmall cell lung cancer. Dual luciferase reporter assay detects the interaction of miR363 with HMGA2. Results The expression of miR363 in nonsmall cell lung cancer tissues was significantly higher than that in adjacent nonsmall cell lung cancer tissues and the highest expression level of miR363 in nonsmall cell lung cancer A549 cells. The inhibitory effect of miR363 on the proliferation of nonsmall cell lung cancer cells attenuated and promoted its apoptosis behavior. The inhibition of miR363 expression inhibited the migration and invasion ability of A549 cells accordingly. miR363 can specifically bind to the 3'UTR of HMGA2 and regulate the activity of HMGA2 expression. Conclusion miR363 plays an important role in the development and progression of nonsmall cell lung cancer and may affect the growth and invasion of nonsmall cell lung cancer cells by regulating the expression of HMGA2.
Key words:  Non small cell lung cancer  miR-363  HMGA2  Invasive behavior

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