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百里醌对大鼠肠缺血再灌注损伤的保护作用
吉祖进,张志云,雷新益,杨勇,蒋学军,庹磊
0
(国药东风总医院)
摘要:
【摘要】 目的 观察百里醌对大鼠肠缺血再灌注损伤(IIRI)的保护作用。方法 45只健康雄性SD大鼠,随机分成3组:假手术对照组(A组)、肠缺血再灌注组(B组)和肠缺血再灌注+百里醌组(C组)。B组和C组分别建立大鼠肠缺血再灌注模型,C组在再灌注同时腹腔注射百里醌(50mg/kg )。方法 HE染色观察小肠组织形态学改变;测定丙二醛(MDA)、超氧化物岐化酶(SOD)含量;采用原位缺口末端标记检测肠黏膜上皮细胞凋亡指数(AI);RTPCR检测小肠组织中的caspase3、Bax和Bcl2的表达情况。结果 B组可见肠绒毛排列紊乱,肠黏膜坏死等表现,经百里醌治疗后明显改善;A组、B组和C组的凋亡指数分别为(453±028)%、(2173±117)%、(953±096)%,SOD活性分别为(13537±334)、(7645±139)和(9513±164) U/mg蛋白,MDA含量分别为(283±036)、(923±078)和(497±045) nmol/mg蛋白;与A组比较,B组中Bax、caspase3 mRNA表达水平明显增高,Bcl2表达水平降低 (P<005)。与B组比较,C组中Bcl2表达水平增高,Bax、caspase3表达水平减低(P<0.05)。结论 百里醌可减轻氧化应激水平,发挥抗凋亡作用, 从而在肠IIRI过程中发挥保护作用。
关键词:  百里醌  缺血再灌注损伤  
DOI:
基金项目:
Protective effects of thymoquinone on intestinal ischemia reperfusion injury in rats
JI Zujin,ZHANG Zhiyun,LEI Xinyi,YANG Yong,JIANG Xueju,TUO Lei
(Dongfeng General Hospital of Chinese Medicine)
Abstract:
【Abstract】 Objective To investigate the protective effect of thymoquinone on intestinal ischemia reperfusion injury in rats. Methods Fortyfive male SD rats were randomly divided into Shamoperation control group (group A), ischemia reperfusion group (group B) and ischemia reperfusion+thymoquinone group (group C). Intestinal ischemia reperfusion model was established in group B and C. Thymoquinone (50 mg /kg ) was injected intraperitoneally followed reperfusion in group C. The pathological changes of the intestine were observed by HE staining. MDA and SOD content were determined. Cell apoptosis index was detected by dUTP nick end labeling. The expression of caspase3, Bax and Bcl2 in intestinal tissue were detected by RTPCR. Results Group B showed intestinal villus disorderly arrangement, intestinal mucosa epithelia necrosis thymoquinone treatment significantly improved the above changes. AI in group A,group B and group C were (453±028)%, (2173±117)% and (953±096)%, respectively. The SOD activities were (13537±334), (7645±139) and (9513±164)U/mg, respectively. MDA were (283±036), (923±078) and (497±045)nmol/mg. Compared with group A, the mRNA expression of Bax and caspase3 were significantly increased, and the mRNA expression of Bcl2 was decreased in group B (P<005). Compared with group B, the expression of Bcl2 in was obvious increased, and the expression of Bax and caspase3 decreased in group C(P<005). Conclusion Thymoquinone reduces the level of oxidative stress and inhibits the apoptosis. Thus, it plays a protective role in the process of intestinal ischemiareperfusion injury.
Key words:  Thymoquinone  Ischemia reperfusion injury  Intestine

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