引用本文:[点击复制]
[点击复制]
【打印本页】 【在线阅读全文】【下载PDF全文】 查看/发表评论下载PDF阅读器关闭

←前一篇|后一篇→

过刊浏览    高级检索

本文已被:浏览 295次   下载 51 本文二维码信息
码上扫一扫!
分枝杆菌病噬菌体33D基因组学特征及抗结核潜力
邬亭亭,魏强,郭述良,刘平,罗永艾
0
(成都市第三人民医院呼吸内科;成都市天府新区人民医院内二科;重庆医科大学附属第一医院呼吸与危重症医学科;重庆市长寿区人民医院呼吸内科)
摘要:
【摘要】 目的 研究分枝杆菌噬菌体33D的基因组特性并探索其抗结核治疗的潜力。方法 通过鸟枪法完成33D全基因组测序;使用 DNAStar、TRF、Promoter predictions、tRNAscanSE、Glimmer、BLAST 软件分别对33D基因组进行分析。结果 噬菌体33D基因组为线性双链DNA,全长50036bp;含有84个推定基因,17个已知功能的基因。其中噬菌体DNA复制相关基因:gene6、7、8、9和gene 14;裂解酶基因:gene 47和gene 48。结论 噬菌体33D为烈性噬菌体,具有抗结核潜力,未发现致病基因,可作为“鸡尾酒疗法”的候选噬菌体。
关键词:  分枝杆菌噬菌体33D  基因组  结核分枝杆菌
DOI:
基金项目:国家自然科学基金面上项目(81570010);四川省卫计委科研课题(17PJ381)
Genetic information of mycobacteriophage 33D and the potentials in control of tuberculosis
WU Tingting,WEI Qiang,GUO Shuliang,LIU Ping,LUO Yongai
(Department of Respiratory Medicine,The Third People’s Hospital of Chengdu;Department of Internal Medicine,Chengdu Tianfu New District People's Hospital;Department of Respiratory and Critical Care Medicine,The First Affiliated Hospital of Chongqing Medical University;Department of Respiratory Medicine,The People’s Hospital of Changshou District)
Abstract:
【Abstract】 Objective To explore the genetic information of phage 33D and its antituberculosis potentials. Methods The genome of phage 33D were sequenced. The gene annotations of those genomes were completed by DNAStar, TRF, Promoter predictions, tRNAscanSE, Glimmer and BLAST. Results The genome of phage 33D was a dsDNA sequence of 50036 bp. The genome of phage 33D encodes 84 gene, 17 gene functions had been identified by BLAST comparison. We propose that gene47 and gene 48 could encode lyases. We proposed that gene6, 7, 8, 9 and 14 were related to DNA Replication . Conclusion 33D is lytic phage which have potentials in control of tuberculosis and have not virulence gene. Phage 33D could be used in phage cocktail therapy.
Key words:  Mycobacteriophage 33D  Genome  Mycobacterium tuberculosis

用微信扫一扫

用微信扫一扫