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抑制糖原合酶激酶活性对老龄大鼠内皮祖细胞增殖的作用机制
崔斌,刘曦,秦浙学,陈剑飞,李佳蓓,于世勇,黄岚
0
(第三军医大学新桥医院全军心血管内科研究所)
摘要:
【摘要】 目的 探讨抑制糖原合酶激酶3β(GSK3β)活性对老龄大鼠内皮祖细胞(EPC)增殖的作用机制。 方法 密度梯度离心法分离培养老龄大鼠骨髓源性EPC,取对数生长期的EPC,分别加入表达催化GSK3β失活的GSK3βKM基因的重组缺陷型腺病毒pMSCVGSK3βKM(基因转染组)或空病毒pMSCVGFP(对照组)。采用镜下计数法及四氮唑溴盐比色法(MTT)测定EPC增殖能力,荧光激活细胞分离仪(FACS)分析各组EPC细胞周期变化。采用蛋白印迹法测定Wnt信号通路中磷酸化糖原合酶激酶3β(pGSK3β)、β连环蛋白(βcatenin)及细胞周期蛋白D1(cyclinD1)的蛋白表达。 结果 与对照组比较,基因转染组EPC数量显著增加(P<001);MTT法检测基因转染组EPC在490 nm 吸光度值与对照组比较差异有统计学意义(P<001);FACS分析显示基因转染组细胞周期S期比例较对照组显著增加(P<001);蛋白印迹法测定显示,与对照组比较,基因转染组pGSK3β、βcatenin及cyclinD1的蛋白表达显著增加(P<001)。 结论 抑制EPC的GSK3β活性可通过激活Wnt信号通路促进老龄大鼠EPC的增殖能力。
关键词:  糖原合酶激酶3β  内皮祖细胞  增殖  老龄大鼠  基因转染
DOI:
基金项目:国家自然科学基金
The mechanism of glycogen synthase kinase 3β inhibition on the proliferation of aging endothelial progenitor cells
CUI Bin,LIU Xi,QIN Zhexue,CHEN Jianfei,LI Jiapei,YU Shiyong,HUANG Lan
(Institute of Cardiovascular Disease of PLA, Xinqiao Hospital, The Third Military Medical University)
Abstract:
【Abstract】 Objective To investigate the mechanism of glycogen synthase kinase 3β(GSK3β)inhibition on the proliferation of aging endothelial progenitor cells(EPCs).Methods Mononuclear cells (MNCs) were isolated from bone marrow in aging Wister rats by density gradient centrifugation combined. EPCs were cultured and transduced with replication defective adenovirus vector expressing catalytically inactive glycogen synthase kinase 3β(GKS3βKM) or green fluorescent protein. EPCs proliferation was assessed by cells count and 3{4,5dimethylthiazol2yl}2,5diphenyltetrazolium bromide (MTT)assay. The cell cycle of EPCs was measured by fluorescenceactivated cell sorting (FACS). The expression of phosphorglycogen synthase kinase 3β(pGSK3β), βcatenin and cyclinD1 in EPCs were detected by western blot. Results GSK3β inhibition improved aging EPCs proliferation. Compared with control group, EPCs proliferation was enhanced in GKS3βKM gene transfection group (GSKi group). The number of EPCs was obviously increased in GSKi groups than that in control group. S phrase in cell cycle was increased in GSKi group in comparison with control group by FACS assay. The protein expression of pGSK3β,βcatenin and cyclinD1 were significantly higher than that in control group. Conclusion GSK3β inhibition could improve aging EPCs proliferation by activating Wnt signal pathway.
Key words:  Glycogen synthase kinase 3β  Endothelial progenitor cell  Proliferation  Aging  Gene transfection

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