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白介素-8在原发性痛风性关节炎患者外周血的表达及意义
钟晓武,姚承佼,周京国,何泳龙,姚亚希,彭元洪,杨其彬,青玉凤,方莉,谢文光
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(川北医学院附属医院检验科;川北医学院附属医院风湿免疫科;川北医学院附属医院医学研究中心)
摘要:
【摘要】目的 分析白介素-8(IL-8)在痛风性关节炎(GA)患者外周血中的表达变化,探讨其临床意义。方法 选择急性痛风性关节炎(AGA组)、非急性痛风性关节炎间歇期(NAGA组) 和健康体检者(HC组) 各43 例。利用RTPCR和Western blot 方法分别检测外周血单个核细胞(PBMCs)中 IL8 mRNA和蛋白表达,再用ELISA法检测各组血浆IL8水平。结果 IL-8 mRNA在AGA 组PBMCs中的表达高于NAGA组和HC组(均P<0.01),在NAGA组的表达高于HC组(P<0.01)。PBMCs 中的IL-8 蛋白水平在AGA 组高于NAGA组和HC组 (均P<0.01),且HC组最低,但NAGA组和HC组比较差异无统计学意义(P>005)。血浆中IL8水平在AGA 组和NAGA组显著高于HC组 (均P<0.01),NAGA组显著高于HC组(P< 0.05 )。结论〓IL8在GA患者的外周血中异常表达,提示IL8在GA的病理机制中可能发挥重要的作用。
关键词:  痛风性关节炎  外周血  炎症  IL-8
DOI:
基金项目:四川省科技厅面上项目(2017JY0151,2017JY0037);四川省卫生和计划生育委员会科研课题(16PJ125);四川省教育厅科研项目(16CZ0023)
Expression and significance of IL-8 in patients with primary gouty arthritis
ZHONG Xiaowu,YAO Chengjiao,ZHOU Jingguo,HE Yonglong,Yao Yaxi,PENG Yuanhong,YANG Qibin,QING Yufeng,FANG Li,XIE Wenguang
(Department of Clinical Laboratory, The Affiliated Hospital of North Sichuan Medical College;Department of Rheumatology and Immunology;Medical Research Center, The Affiliated Hospital of North Sichuan Medical College)
Abstract:
【Abstract】Objective To analyze the expression and clinical significance of IL8 in peripheral blood in patients with gouty arthritis (GA). Methods The peripheral blood were collected from 43 patients with acute gouty arthritis (AGA), 43 patients with nonacute gouty arthritis (NAGA) and 43 healthy controls (HC). The expression levels of IL8 mRNA and protein in peripheral blood mononuclear cells (PBMCs) were detected by realtime fluorescent quantitative polymerase chain reaction and Western blot. The protien expressions of IL8 were measured by enzyme linked immunosorbent assay (ELISA) in plasma. Results The expression of IL8 mRNA in PBMCs of the AGA group was significantly higher than that of the IGA and HC groups (all P<0.01), and IGA group higher than HC groups (P<0.01). Compared with IGA and HC groups, the protein expression of IL8 significantly increased in PBMCs of the AGA group, and the HC group was the lowest.There were no statistical differences between IGA and HC groups. The expression of IL8 was significantly higher in plasma of the AGA group compared with the IGA and HC groups (all P<0.01), and IGA group significantly higher than HC groups (P<0.05).Conclusion The expression of IL8 changes significantly in GA patients in comparison with HC, which implies that IL8 may have an important role in progression of GA.
Key words:  Gouty arthritis  Peripheral blood  Inflammation  IL-8

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