引用本文:[点击复制]
[点击复制]
【打印本页】 【在线阅读全文】【下载PDF全文】 查看/发表评论下载PDF阅读器关闭

←前一篇|后一篇→

过刊浏览    高级检索

本文已被:浏览 113次   下载 5 本文二维码信息
码上扫一扫!
长链非编码RNA HOTAIR在子宫内膜癌表达及其对子宫内膜癌细胞增殖迁移及侵袭能力的影响
王丹丹,王焱,张俊俊,杜趁香
0
(河南科技大学临床医学院·河南科技大学第一附属医院)
摘要:
【摘要】目的 研究长链非编码 RNA(long non coding RNA, lncRNA)HOX转录反义RNA(HOX transcript antisense RNA, HOTAIR)对子宫内膜癌细胞增殖、迁移和侵袭能力的影响。方法 利用定量反转录PCR(qRT PCR)检测 HOTAIR在20例子宫内膜癌组织及癌旁组织的表达。通过MTT法和Transwell法研究HOTAIR对子宫内膜癌Ishikawa细胞增殖、迁移及侵袭能力的影响。结果 相比癌旁组织,HOTAIR在子宫内膜癌组织表达水平升高。转染HOTAIR siRNA 36 h后,Ishikawa细胞中HOTAIR表达下调;转染pcDNA3.1 HOTAIR 36 h后,Ishikawa细胞中HOTAIR表达上调。MTT实验结果显示,抑制HOTAIR的表达能够降低Ishikawa细胞的增殖能力,上调HOTAIR的表达则增强Ishikawa细胞的增殖能力。Transwell实验结果显示,下调HOTAIR表达可抑制癌细胞的迁移和侵袭;过表达HOTAIR则促进Ishikawa细胞迁移、侵袭。 结论 LncRNA HOTAIR在子宫内膜癌中高表达,下调HOTAIR表达能够抑制子宫内膜癌Ishikawa细胞增殖、迁移和侵袭能力。
关键词:  子宫内膜癌  长链非编码RNA  HOTAIR  细胞增殖  迁移  侵袭
DOI:
基金项目:
Expression of long chain noncoding RNA HOTAIR in endometrial cancer and its effects on proliferation, migration and invasion of endometrial Cancer cells
WANG Dandan,WANG Yan,ZHANG Junjun,DU chenxiang
(College of Clinical Medicine, The First Affiliated Hospital of Henan University of Science and Technology)
Abstract:
【Abstract】Objective To investigate the effects of long noncoding RNA HOX transcript antisense RNA (HOTAIR) on proliferation, migration and invasion of endometrial carcinoma cells. Methods Quantitative reverse transcription PCR (qRTPCR) was used to detect the expression of HOTAIR in 20 cases of endometrial cancer and adjacent tissues. The effects of HOTAIR on proliferation, migration and invasion of endometrial cancer Ishikawa cells were studied by MTT and transwell experiments. Results The expression level of HOTAIR in endometrial cancer tissues was higher than that in adjacent tissues. At 36 h after transfection with HOTAIR siRNA, expression of HOTAIR was decreased in Ishikawa cells. At 36 h after transfection with pcDNA3.1 HOTAIR, expression of HOTAIR was increased in Ishikawa cells. The results of MTT experiments showed that inhibition of HOTAIR expression could decrease the proliferation ability of Ishikawa cells, and HOTAIR overexpression could enhance the proliferation ability of Ishikawa cells. The results of transwell experiments demonstrated that downregulation of HOTAIR expression could inhibit the migration and invasion of Ishikawa cells, and HOTAIR overexpression could promote the migration and invasion of Ishikawa cells. Conclusion LncRNA HOTAIR is highly expressed in endometrial cancer. Down regulation of HOTAIR could inhibit the proliferation, migration and invasion of endometrial cancer Ishikawa cells.
Key words:  Endometrial cancer  Long non coding RNA  HOTAIR  Proliferation  Migration  Invasion

用微信扫一扫

用微信扫一扫